Construct: shRNA TRCN0000274229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007111.4-557s21c1
DNA Barcode:
CCTACGGCATTTCTCCATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TFDP1 (7027)
Gene Description:
transcription factor Dp-1
Transcript:
RefSeq NM_007111.4 (NON-CURRENT)
Match location:
Position 557 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGGCATTTCTCCATGAA
Hairpin Sequence:
5'-CCGG-CCTACGGCATTTCTCCATGAA-CTCGAG-TTCATGGAGAAATGCCGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGGCATTTCTCCATGAACTCGAGTTCATGGAGAAATGCCGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGGCATTTCTCCATGAACTCGAGTTCATGGAGAAATGCCGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000019880