Construct: shRNA TRCN0000274359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012081.5-911s21c1
DNA Barcode:
CCCTGCAAATACAATTCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELL2 (22936)
Gene Description:
elongation factor for RNA polymerase II 2
Transcript:
RefSeq NM_012081.5 (NON-CURRENT)
Match location:
Position 911 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCAAATACAATTCGAAA
Hairpin Sequence:
5'-CCGG-CCCTGCAAATACAATTCGAAA-CTCGAG-TTTCGAATTGTATTTGCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCAAATACAATTCGAAACTCGAGTTTCGAATTGTATTTGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCAAATACAATTCGAAACTCGAGTTTCGAATTGTATTTGCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000017912