Construct: shRNA TRCN0000274404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020432.4-231s21c1
DNA Barcode:
GCTAAGGAATAAACCAAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHTF2 (57157)
Gene Description:
putative homeodomain transcription factor 2
Transcript:
RefSeq NM_020432.4 (NON-CURRENT)
Match location:
Position 231 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAGGAATAAACCAAAGAA
Hairpin Sequence:
5'-CCGG-GCTAAGGAATAAACCAAAGAA-CTCGAG-TTCTTTGGTTTATTCCTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAGGAATAAACCAAAGAACTCGAGTTCTTTGGTTTATTCCTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAGGAATAAACCAAAGAACTCGAGTTCTTTGGTTTATTCCTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000017180