Construct: shRNA TRCN0000274491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008824.2-1121s21c1
DNA Barcode:
CTACGGGACCAGGATAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pfkfb1 (18639)
Gene Description:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Transcript:
RefSeq NM_008824.2 (NON-CURRENT)
Match location:
Position 1121 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACGGGACCAGGATAAATAT
Hairpin Sequence:
5'-CCGG-CTACGGGACCAGGATAAATAT-CTCGAG-ATATTTATCCTGGTCCCGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACGGGACCAGGATAAATATCTCGAGATATTTATCCTGGTCCCGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACGGGACCAGGATAAATATCTCGAGATATTTATCCTGGTCCCGTAG-3'

Other clones with same target sequence:

(none)