Construct: shRNA TRCN0000274518

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018869.3-2400s21c1
DNA Barcode:
GCTGAATGTGTTCGGACCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grk5 (14773)
Gene Description:
G protein-coupled receptor kinase 5
Transcript:
RefSeq NM_018869.3 (CURRENT)
Match location:
Position 2400 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274518)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATGTGTTCGGACCTAA
Hairpin Sequence:
5'-CCGG-GCTGAATGTGTTCGGACCTAA-CTCGAG-TTAGGTCCGAACACATTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATGTGTTCGGACCTAACTCGAGTTAGGTCCGAACACATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATGTGTTCGGACCTAACTCGAGTTAGGTCCGAACACATTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000022832