Construct: shRNA TRCN0000274540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010690.1-2734s21c1
DNA Barcode:
GGGATATGATGAGCCTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lats1 (16798)
Gene Description:
large tumor suppressor
Transcript:
RefSeq NM_010690.1 (CURRENT)
Match location:
Position 2734 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGATATGATGAGCCTATTAA
Hairpin Sequence:
5'-CCGG-GGGATATGATGAGCCTATTAA-CTCGAG-TTAATAGGCTCATCATATCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGATATGATGAGCCTATTAACTCGAGTTAATAGGCTCATCATATCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGATATGATGAGCCTATTAACTCGAGTTAATAGGCTCATCATATCCC-3'

Other clones with same target sequence:

(none)