Construct: shRNA TRCN0000274652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011163.3-1862s21c1
DNA Barcode:
GTGACCCACAGACATTGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif2ak2 (19106)
Gene Description:
eukaryotic translation initiation factor 2-alpha kinase 2
Transcript:
RefSeq NM_011163.3 (NON-CURRENT)
Match location:
Position 1862 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACCCACAGACATTGTATT
Hairpin Sequence:
5'-CCGG-GTGACCCACAGACATTGTATT-CTCGAG-AATACAATGTCTGTGGGTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACCCACAGACATTGTATTCTCGAGAATACAATGTCTGTGGGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACCCACAGACATTGTATTCTCGAGAATACAATGTCTGTGGGTCAC-3'

Other clones with same target sequence:

(none)