Construct: shRNA TRCN0000274738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013861.3-1022s21c1
DNA Barcode:
ATTCCAGGTAATGCTATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpk1 (29807)
Gene Description:
thiamine pyrophosphokinase
Transcript:
RefSeq NM_013861.3 (NON-CURRENT)
Match location:
Position 1022 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCCAGGTAATGCTATAAAT
Hairpin Sequence:
5'-CCGG-ATTCCAGGTAATGCTATAAAT-CTCGAG-ATTTATAGCATTACCTGGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCCAGGTAATGCTATAAATCTCGAGATTTATAGCATTACCTGGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCCAGGTAATGCTATAAATCTCGAGATTTATAGCATTACCTGGAAT-3'

Other clones with same target sequence:

(none)