Construct: shRNA TRCN0000274748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016695.3-573s21c1
DNA Barcode:
GAACACCTGGGTGTGACATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mpp2 (50997)
Gene Description:
membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Transcript:
RefSeq NM_016695.3 (CURRENT)
Match location:
Position 573 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACACCTGGGTGTGACATTC
Hairpin Sequence:
5'-CCGG-GAACACCTGGGTGTGACATTC-CTCGAG-GAATGTCACACCCAGGTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACACCTGGGTGTGACATTCCTCGAGGAATGTCACACCCAGGTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACACCTGGGTGTGACATTCCTCGAGGAATGTCACACCCAGGTGTTC-3'

Other clones with same target sequence:

(none)