Construct: shRNA TRCN0000274749
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_016695.3-2261s21c1
- DNA Barcode:
- CTAGGCAGACCCAGGTAATAG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mpp2 (50997)
- Gene Description:
- membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
- Transcript:
- RefSeq NM_016695.3 (CURRENT)
- Match location:
- Position 2261 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000274749)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTAGGCAGACCCAGGTAATAG
- Hairpin Sequence:
-
5'-CCGG-CTAGGCAGACCCAGGTAATAG-CTCGAG-CTATTACCTGGGTCTGCCTAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTAGGCAGACCCAGGTAATAGCTCGAGCTATTACCTGGGTCTGCCTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTAGGCAGACCCAGGTAATAGCTCGAGCTATTACCTGGGTCTGCCTAG-3'
Other clones with same target sequence:
(none)
