Construct: shRNA TRCN0000274768

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021420.3-1212s21c1
DNA Barcode:
CCGTCTTTCCTTGAATACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk4 (58231)
Gene Description:
serine/threonine kinase 4
Transcript:
RefSeq NM_021420.3 (CURRENT)
Match location:
Position 1212 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274768)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCTTTCCTTGAATACTTT
Hairpin Sequence:
5'-CCGG-CCGTCTTTCCTTGAATACTTT-CTCGAG-AAAGTATTCAAGGAAAGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCTTTCCTTGAATACTTTCTCGAGAAAGTATTCAAGGAAAGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCTTTCCTTGAATACTTTCTCGAGAAAGTATTCAAGGAAAGACGG-3'

Other clones with same target sequence:

  1. TRCN0000025286