Construct: shRNA TRCN0000274819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024721.3-4857s21c1
DNA Barcode:
TGATCCATCTCGTCCATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFHX4 (79776)
Gene Description:
zinc finger homeobox 4
Transcript:
RefSeq NM_024721.3 (NON-CURRENT)
Match location:
Position 4857 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCCATCTCGTCCATATAA
Hairpin Sequence:
5'-CCGG-TGATCCATCTCGTCCATATAA-CTCGAG-TTATATGGACGAGATGGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCCATCTCGTCCATATAACTCGAGTTATATGGACGAGATGGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCCATCTCGTCCATATAACTCGAGTTATATGGACGAGATGGATCA-3'

Other clones with same target sequence:

(none)