Construct: shRNA TRCN0000274837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057179.1-563s21c1
DNA Barcode:
CTTCTCCGTGTGGCGCATGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TWIST2 (117581)
Gene Description:
twist family bHLH transcription factor 2
Transcript:
RefSeq NM_057179.1 (NON-CURRENT)
Match location:
Position 563 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTCCGTGTGGCGCATGGA
Hairpin Sequence:
5'-CCGG-CTTCTCCGTGTGGCGCATGGA-CTCGAG-TCCATGCGCCACACGGAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTCCGTGTGGCGCATGGACTCGAGTCCATGCGCCACACGGAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTCCGTGTGGCGCATGGACTCGAGTCCATGCGCCACACGGAGAAG-3'

Other clones with same target sequence:

  1. TRCN0000020873