Construct: shRNA TRCN0000274885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032495.4-420s21c1
DNA Barcode:
GACCCAGAAATGGTTTAAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HOPX (84525)
Gene Description:
HOP homeobox
Transcript:
RefSeq NM_032495.4 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000274885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCAGAAATGGTTTAAGCA
Hairpin Sequence:
5'-CCGG-GACCCAGAAATGGTTTAAGCA-CTCGAG-TGCTTAAACCATTTCTGGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCAGAAATGGTTTAAGCACTCGAGTGCTTAAACCATTTCTGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCAGAAATGGTTTAAGCACTCGAGTGCTTAAACCATTTCTGGGTC-3'

Other clones with same target sequence:

  1. TRCN0000016407