Construct: shRNA TRCN0000275018

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052879.3-2363s21c1
DNA Barcode:
ACTTGAACTGTGGCTATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LARP4 (113251)
Gene Description:
La ribonucleoprotein 4
Transcript:
RefSeq NM_052879.3 (NON-CURRENT)
Match location:
Position 2363 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275018)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGAACTGTGGCTATATTG
Hairpin Sequence:
5'-CCGG-ACTTGAACTGTGGCTATATTG-CTCGAG-CAATATAGCCACAGTTCAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGAACTGTGGCTATATTGCTCGAGCAATATAGCCACAGTTCAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGAACTGTGGCTATATTGCTCGAGCAATATAGCCACAGTTCAAGT-3'

Other clones with same target sequence:

(none)