Construct: shRNA TRCN0000275028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018684.1-1125s21c1
DNA Barcode:
GCCCATATCTTGTTTCAGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC4H2 (55906)
Gene Description:
zinc finger C4H2-type containing
Transcript:
RefSeq NM_018684.1 (NON-CURRENT)
Match location:
Position 1125 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATATCTTGTTTCAGGTA
Hairpin Sequence:
5'-CCGG-GCCCATATCTTGTTTCAGGTA-CTCGAG-TACCTGAAACAAGATATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATATCTTGTTTCAGGTACTCGAGTACCTGAAACAAGATATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATATCTTGTTTCAGGTACTCGAGTACCTGAAACAAGATATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000128001