Construct: shRNA TRCN0000275042

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152266.3-869s21c1
DNA Barcode:
CGCCTCTAATCTCAGCACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAAP24 (91442)
Gene Description:
FA core complex associated protein 24
Transcript:
RefSeq NM_152266.3 (NON-CURRENT)
Match location:
Position 869 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275042)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTCTAATCTCAGCACTTT
Hairpin Sequence:
5'-CCGG-CGCCTCTAATCTCAGCACTTT-CTCGAG-AAAGTGCTGAGATTAGAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTCTAATCTCAGCACTTTCTCGAGAAAGTGCTGAGATTAGAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTCTAATCTCAGCACTTTCTCGAGAAAGTGCTGAGATTAGAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000138644