Construct: shRNA TRCN0000275050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145753.2-3937s21c1
DNA Barcode:
ACTCATGACAGAATCTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHLDB2 (90102)
Gene Description:
pleckstrin homology like domain family B member 2
Transcript:
RefSeq NM_145753.2 (CURRENT)
Match location:
Position 3937 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCATGACAGAATCTATTAT
Hairpin Sequence:
5'-CCGG-ACTCATGACAGAATCTATTAT-CTCGAG-ATAATAGATTCTGTCATGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCATGACAGAATCTATTATCTCGAGATAATAGATTCTGTCATGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCATGACAGAATCTATTATCTCGAGATAATAGATTCTGTCATGAGT-3'

Other clones with same target sequence:

(none)