Construct: shRNA TRCN0000275165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025194.2-750s21c1
DNA Barcode:
TCCCTGGACAGAGCCATATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITPKC (80271)
Gene Description:
inositol-trisphosphate 3-kinase C
Transcript:
RefSeq NM_025194.2 (NON-CURRENT)
Match location:
Position 750 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCTGGACAGAGCCATATAC
Hairpin Sequence:
5'-CCGG-TCCCTGGACAGAGCCATATAC-CTCGAG-GTATATGGCTCTGTCCAGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCTGGACAGAGCCATATACCTCGAGGTATATGGCTCTGTCCAGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCTGGACAGAGCCATATACCTCGAGGTATATGGCTCTGTCCAGGGA-3'

Other clones with same target sequence:

(none)