Construct: shRNA TRCN0000275175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024729.3-6150s21c1
DNA Barcode:
GTGCAGCACTCTGGCATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYH14 (79784)
Gene Description:
myosin heavy chain 14
Transcript:
RefSeq NM_024729.3 (CURRENT)
Match location:
Position 6150 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCAGCACTCTGGCATTTAT
Hairpin Sequence:
5'-CCGG-GTGCAGCACTCTGGCATTTAT-CTCGAG-ATAAATGCCAGAGTGCTGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCAGCACTCTGGCATTTATCTCGAGATAAATGCCAGAGTGCTGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCAGCACTCTGGCATTTATCTCGAGATAAATGCCAGAGTGCTGCAC-3'

Other clones with same target sequence:

(none)