Construct: shRNA TRCN0000275231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018096.3-277s21c1
DNA Barcode:
GACAGAGAAGGTCCTAGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLE1 (54475)
Gene Description:
notchless homolog 1
Transcript:
RefSeq NM_018096.3 (NON-CURRENT)
Match location:
Position 277 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGAGAAGGTCCTAGACAT
Hairpin Sequence:
5'-CCGG-GACAGAGAAGGTCCTAGACAT-CTCGAG-ATGTCTAGGACCTTCTCTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGAGAAGGTCCTAGACATCTCGAGATGTCTAGGACCTTCTCTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGAGAAGGTCCTAGACATCTCGAGATGTCTAGGACCTTCTCTGTC-3'

Other clones with same target sequence:

  1. TRCN0000154710