Construct: shRNA TRCN0000275241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001755.2-721s21c1
DNA Barcode:
GAAGATAGAGACAGGTCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBFB (865)
Gene Description:
core-binding factor subunit beta
Transcript:
RefSeq NM_001755.2 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATAGAGACAGGTCTCAT
Hairpin Sequence:
5'-CCGG-GAAGATAGAGACAGGTCTCAT-CTCGAG-ATGAGACCTGTCTCTATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATAGAGACAGGTCTCATCTCGAGATGAGACCTGTCTCTATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATAGAGACAGGTCTCATCTCGAGATGAGACCTGTCTCTATCTTC-3'

Other clones with same target sequence:

  1. TRCN0000016645