Construct: shRNA TRCN0000275338

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016213.3-1271s21c1
DNA Barcode:
GGACTAGAGTTCAACTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIP4 (9325)
Gene Description:
thyroid hormone receptor interactor 4
Transcript:
RefSeq NM_016213.3 (NON-CURRENT)
Match location:
Position 1271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275338)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACTAGAGTTCAACTCATTT
Hairpin Sequence:
5'-CCGG-GGACTAGAGTTCAACTCATTT-CTCGAG-AAATGAGTTGAACTCTAGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACTAGAGTTCAACTCATTTCTCGAGAAATGAGTTGAACTCTAGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACTAGAGTTCAACTCATTTCTCGAGAAATGAGTTGAACTCTAGTCC-3'

Other clones with same target sequence:

(none)