Construct: shRNA TRCN0000275409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138348.4-659s21c1
DNA Barcode:
GCAATGGAAACTTGGACTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OTULIN (90268)
Gene Description:
OTU deubiquitinase with linear linkage specificity
Transcript:
RefSeq NM_138348.4 (NON-CURRENT)
Match location:
Position 659 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGGAAACTTGGACTGAA
Hairpin Sequence:
5'-CCGG-GCAATGGAAACTTGGACTGAA-CTCGAG-TTCAGTCCAAGTTTCCATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGGAAACTTGGACTGAACTCGAGTTCAGTCCAAGTTTCCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGGAAACTTGGACTGAACTCGAGTTCAGTCCAAGTTTCCATTGC-3'

Other clones with same target sequence:

  1. TRCN0000135471