Construct: shRNA TRCN0000275422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017929.4-786s21c1
DNA Barcode:
CTGGATGTACTTCAGGCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PEX26 (55670)
Gene Description:
peroxisomal biogenesis factor 26
Transcript:
RefSeq NM_017929.4 (NON-CURRENT)
Match location:
Position 786 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATGTACTTCAGGCCATT
Hairpin Sequence:
5'-CCGG-CTGGATGTACTTCAGGCCATT-CTCGAG-AATGGCCTGAAGTACATCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATGTACTTCAGGCCATTCTCGAGAATGGCCTGAAGTACATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATGTACTTCAGGCCATTCTCGAGAATGGCCTGAAGTACATCCAG-3'

Other clones with same target sequence:

  1. TRCN0000157635