Construct: shRNA TRCN0000275465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018252.2-735s21c1
DNA Barcode:
GAACAAGAGTAGTGAGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PACC1 (55248)
Gene Description:
proton activated chloride channel 1
Transcript:
RefSeq NM_018252.2 (NON-CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAAGAGTAGTGAGGACTT
Hairpin Sequence:
5'-CCGG-GAACAAGAGTAGTGAGGACTT-CTCGAG-AAGTCCTCACTACTCTTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAAGAGTAGTGAGGACTTCTCGAGAAGTCCTCACTACTCTTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAAGAGTAGTGAGGACTTCTCGAGAAGTCCTCACTACTCTTGTTC-3'

Other clones with same target sequence:

  1. TRCN0000136154