Construct: shRNA TRCN0000275479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006624.3-2214s21c1
DNA Barcode:
ACATCAGTGGAGGGTATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMYND11 (10771)
Gene Description:
zinc finger MYND-type containing 11
Transcript:
RefSeq NM_006624.3 (NON-CURRENT)
Match location:
Position 2214 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATCAGTGGAGGGTATTATT
Hairpin Sequence:
5'-CCGG-ACATCAGTGGAGGGTATTATT-CTCGAG-AATAATACCCTCCACTGATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATCAGTGGAGGGTATTATTCTCGAGAATAATACCCTCCACTGATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATCAGTGGAGGGTATTATTCTCGAGAATAATACCCTCCACTGATGT-3'

Other clones with same target sequence:

(none)