Construct: shRNA TRCN0000275489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014676.2-3087s21c1
DNA Barcode:
GCGTTTAAGGGACAGGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PUM1 (9698)
Gene Description:
pumilio RNA binding family member 1
Transcript:
RefSeq NM_014676.2 (NON-CURRENT)
Match location:
Position 3087 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTTAAGGGACAGGTATTT
Hairpin Sequence:
5'-CCGG-GCGTTTAAGGGACAGGTATTT-CTCGAG-AAATACCTGTCCCTTAAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTTAAGGGACAGGTATTTCTCGAGAAATACCTGTCCCTTAAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTTAAGGGACAGGTATTTCTCGAGAAATACCTGTCCCTTAAACGC-3'

Other clones with same target sequence:

(none)