Construct: shRNA TRCN0000275554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000964.2-594s21c1
DNA Barcode:
CAGCTTCCAGTTAGTGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RARA (5914)
Gene Description:
retinoic acid receptor alpha
Transcript:
RefSeq NM_000964.2 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTTCCAGTTAGTGGATAT
Hairpin Sequence:
5'-CCGG-CAGCTTCCAGTTAGTGGATAT-CTCGAG-ATATCCACTAACTGGAAGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTTCCAGTTAGTGGATATCTCGAGATATCCACTAACTGGAAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTTCCAGTTAGTGGATATCTCGAGATATCCACTAACTGGAAGCTG-3'

Other clones with same target sequence:

  1. TRCN0000020373