Construct: shRNA TRCN0000275563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016652.4-1642s21c1
DNA Barcode:
GGATCAACTATGCACTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRNKL1 (51340)
Gene Description:
crooked neck pre-mRNA splicing factor 1
Transcript:
RefSeq NM_016652.4 (NON-CURRENT)
Match location:
Position 1642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCAACTATGCACTCTATG
Hairpin Sequence:
5'-CCGG-GGATCAACTATGCACTCTATG-CTCGAG-CATAGAGTGCATAGTTGATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCAACTATGCACTCTATGCTCGAGCATAGAGTGCATAGTTGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCAACTATGCACTCTATGCTCGAGCATAGAGTGCATAGTTGATCC-3'

Other clones with same target sequence:

(none)