Construct: shRNA TRCN0000275617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006644.2-804s21c1
DNA Barcode:
CCAGTAACAGATTGTGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSPH1 (10808)
Gene Description:
heat shock protein family H (Hsp110) member 1
Transcript:
RefSeq NM_006644.2 (NON-CURRENT)
Match location:
Position 804 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAACAGATTGTGTTATT
Hairpin Sequence:
5'-CCGG-CCAGTAACAGATTGTGTTATT-CTCGAG-AATAACACAATCTGTTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAACAGATTGTGTTATTCTCGAGAATAACACAATCTGTTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAACAGATTGTGTTATTCTCGAGAATAACACAATCTGTTACTGG-3'

Other clones with same target sequence:

  1. TRCN0000160594