Construct: shRNA TRCN0000275629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024782.2-706s21c1
DNA Barcode:
ATTCCTTCTTGGAACAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NHEJ1 (79840)
Gene Description:
non-homologous end joining factor 1
Transcript:
RefSeq NM_024782.2 (CURRENT)
Match location:
Position 706 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCCTTCTTGGAACAATTTA
Hairpin Sequence:
5'-CCGG-ATTCCTTCTTGGAACAATTTA-CTCGAG-TAAATTGTTCCAAGAAGGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCCTTCTTGGAACAATTTACTCGAGTAAATTGTTCCAAGAAGGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCCTTCTTGGAACAATTTACTCGAGTAAATTGTTCCAAGAAGGAAT-3'

Other clones with same target sequence:

(none)