Construct: shRNA TRCN0000275776

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015375.2-2147s21c1
DNA Barcode:
GCTTTGGAATTTCACTATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DSTYK (25778)
Gene Description:
dual serine/threonine and tyrosine protein kinase
Transcript:
RefSeq NM_015375.2 (NON-CURRENT)
Match location:
Position 2147 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275776)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGGAATTTCACTATATG
Hairpin Sequence:
5'-CCGG-GCTTTGGAATTTCACTATATG-CTCGAG-CATATAGTGAAATTCCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGGAATTTCACTATATGCTCGAGCATATAGTGAAATTCCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGGAATTTCACTATATGCTCGAGCATATAGTGAAATTCCAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000196387