Construct: shRNA TRCN0000275804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004757.3-1205s21c1
DNA Barcode:
AGAAGTAGATGTCGGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AIMP1 (9255)
Gene Description:
aminoacyl tRNA synthetase complex interacting multifunctional protein 1
Transcript:
RefSeq NM_004757.3 (CURRENT)
Match location:
Position 1205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTAGATGTCGGAGAAAT
Hairpin Sequence:
5'-CCGG-AGAAGTAGATGTCGGAGAAAT-CTCGAG-ATTTCTCCGACATCTACTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTAGATGTCGGAGAAATCTCGAGATTTCTCCGACATCTACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTAGATGTCGGAGAAATCTCGAGATTTCTCCGACATCTACTTCT-3'

Other clones with same target sequence:

(none)