Construct: shRNA TRCN0000275806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004757.3-807s21c1
DNA Barcode:
GAGAAGAAACTTCGAGTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AIMP1 (9255)
Gene Description:
aminoacyl tRNA synthetase complex interacting multifunctional protein 1
Transcript:
RefSeq NM_004757.3 (CURRENT)
Match location:
Position 807 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGAAACTTCGAGTTGAA
Hairpin Sequence:
5'-CCGG-GAGAAGAAACTTCGAGTTGAA-CTCGAG-TTCAACTCGAAGTTTCTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGAAACTTCGAGTTGAACTCGAGTTCAACTCGAAGTTTCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGAAACTTCGAGTTGAACTCGAGTTCAACTCGAAGTTTCTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000147840