Construct: shRNA TRCN0000275825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015577.1-3123s21c1
DNA Barcode:
CCGCTGCCATTGTTCTCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAI14 (26064)
Gene Description:
retinoic acid induced 14
Transcript:
RefSeq NM_015577.1 (NON-CURRENT)
Match location:
Position 3123 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTGCCATTGTTCTCATTC
Hairpin Sequence:
5'-CCGG-CCGCTGCCATTGTTCTCATTC-CTCGAG-GAATGAGAACAATGGCAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTGCCATTGTTCTCATTCCTCGAGGAATGAGAACAATGGCAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTGCCATTGTTCTCATTCCTCGAGGAATGAGAACAATGGCAGCGG-3'

Other clones with same target sequence:

(none)