Construct: shRNA TRCN0000275845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020830.3-407s21c1
DNA Barcode:
CGATTATCTTCAGCTTGGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDFY1 (57590)
Gene Description:
WD repeat and FYVE domain containing 1
Transcript:
RefSeq NM_020830.3 (NON-CURRENT)
Match location:
Position 407 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATTATCTTCAGCTTGGCCA
Hairpin Sequence:
5'-CCGG-CGATTATCTTCAGCTTGGCCA-CTCGAG-TGGCCAAGCTGAAGATAATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATTATCTTCAGCTTGGCCACTCGAGTGGCCAAGCTGAAGATAATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATTATCTTCAGCTTGGCCACTCGAGTGGCCAAGCTGAAGATAATCG-3'

Other clones with same target sequence:

  1. TRCN0000137552