Construct: shRNA TRCN0000275899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005776.2-501s21c1
DNA Barcode:
ACCTATATGGCATGATCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNIH1 (10175)
Gene Description:
cornichon family AMPA receptor auxiliary protein 1
Transcript:
RefSeq NM_005776.2 (NON-CURRENT)
Match location:
Position 501 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTATATGGCATGATCTATG
Hairpin Sequence:
5'-CCGG-ACCTATATGGCATGATCTATG-CTCGAG-CATAGATCATGCCATATAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTATATGGCATGATCTATGCTCGAGCATAGATCATGCCATATAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTATATGGCATGATCTATGCTCGAGCATAGATCATGCCATATAGGT-3'

Other clones with same target sequence:

(none)