Construct: shRNA TRCN0000275956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006335.2-421s21c1
DNA Barcode:
GCTGGTATCTTGTTGACAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIMM17A (10440)
Gene Description:
translocase of inner mitochondrial membrane 17A
Transcript:
RefSeq NM_006335.2 (NON-CURRENT)
Match location:
Position 421 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000275956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTATCTTGTTGACAAGA
Hairpin Sequence:
5'-CCGG-GCTGGTATCTTGTTGACAAGA-CTCGAG-TCTTGTCAACAAGATACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTATCTTGTTGACAAGACTCGAGTCTTGTCAACAAGATACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTATCTTGTTGACAAGACTCGAGTCTTGTCAACAAGATACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000149047