Construct: shRNA TRCN0000276087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004491.4-4702s21c1
DNA Barcode:
TGAGCTGGCTTGGACCCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP35 (2909)
Gene Description:
Rho GTPase activating protein 35
Transcript:
RefSeq NM_004491.4 (NON-CURRENT)
Match location:
Position 4702 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGCTGGCTTGGACCCATTT
Hairpin Sequence:
5'-CCGG-TGAGCTGGCTTGGACCCATTT-CTCGAG-AAATGGGTCCAAGCCAGCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGCTGGCTTGGACCCATTTCTCGAGAAATGGGTCCAAGCCAGCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGCTGGCTTGGACCCATTTCTCGAGAAATGGGTCCAAGCCAGCTCA-3'

Other clones with same target sequence:

(none)