Construct: shRNA TRCN0000276105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012432.2-305s21c1
DNA Barcode:
AGTTAGAGACATGGGTAATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SETDB1 (9869)
Gene Description:
SET domain bifurcated histone lysine methyltransferase 1
Transcript:
RefSeq NM_012432.2 (NON-CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTAGAGACATGGGTAATAC
Hairpin Sequence:
5'-CCGG-AGTTAGAGACATGGGTAATAC-CTCGAG-GTATTACCCATGTCTCTAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTAGAGACATGGGTAATACCTCGAGGTATTACCCATGTCTCTAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTAGAGACATGGGTAATACCTCGAGGTATTACCCATGTCTCTAACT-3'

Other clones with same target sequence:

(none)