Construct: shRNA TRCN0000276139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002519.2-4441s21c1
DNA Barcode:
AGTGTAGGGAATGGGATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPAT (4863)
Gene Description:
nuclear protein, coactivator of histone transcription
Transcript:
RefSeq NM_002519.2 (NON-CURRENT)
Match location:
Position 4441 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGTAGGGAATGGGATATTG
Hairpin Sequence:
5'-CCGG-AGTGTAGGGAATGGGATATTG-CTCGAG-CAATATCCCATTCCCTACACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGTAGGGAATGGGATATTGCTCGAGCAATATCCCATTCCCTACACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGTAGGGAATGGGATATTGCTCGAGCAATATCCCATTCCCTACACT-3'

Other clones with same target sequence:

(none)