Construct: shRNA TRCN0000276145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003487.2-1884s21c1
DNA Barcode:
TGACATGATCCATAGTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF15 (8148)
Gene Description:
TATA-box binding protein associated factor 15
Transcript:
RefSeq NM_003487.2 (NON-CURRENT)
Match location:
Position 1884 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACATGATCCATAGTGAAAT
Hairpin Sequence:
5'-CCGG-TGACATGATCCATAGTGAAAT-CTCGAG-ATTTCACTATGGATCATGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACATGATCCATAGTGAAATCTCGAGATTTCACTATGGATCATGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACATGATCCATAGTGAAATCTCGAGATTTCACTATGGATCATGTCA-3'

Other clones with same target sequence:

(none)