Construct: shRNA TRCN0000276150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015677.1-778s21c1
DNA Barcode:
GAGCTGTTTGATTGAAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3YL1 (26751)
Gene Description:
SH3 and SYLF domain containing 1
Transcript:
RefSeq NM_015677.1 (NON-CURRENT)
Match location:
Position 778 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTGTTTGATTGAAAGGAA
Hairpin Sequence:
5'-CCGG-GAGCTGTTTGATTGAAAGGAA-CTCGAG-TTCCTTTCAATCAAACAGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTGTTTGATTGAAAGGAACTCGAGTTCCTTTCAATCAAACAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTGTTTGATTGAAAGGAACTCGAGTTCCTTTCAATCAAACAGCTC-3'

Other clones with same target sequence:

  1. TRCN0000167670