Construct: shRNA TRCN0000276158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016016.2-949s21c1
DNA Barcode:
TCTACCCTTTGACGTGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A39 (51629)
Gene Description:
solute carrier family 25 member 39
Transcript:
RefSeq NM_016016.2 (NON-CURRENT)
Match location:
Position 949 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTACCCTTTGACGTGGTAAA
Hairpin Sequence:
5'-CCGG-TCTACCCTTTGACGTGGTAAA-CTCGAG-TTTACCACGTCAAAGGGTAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACCCTTTGACGTGGTAAACTCGAGTTTACCACGTCAAAGGGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACCCTTTGACGTGGTAAACTCGAGTTTACCACGTCAAAGGGTAGA-3'

Other clones with same target sequence:

(none)