Construct: shRNA TRCN0000276163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030571.3-1033s21c1
DNA Barcode:
CTATTTGTTCCTAGGTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDFIP1 (80762)
Gene Description:
Nedd4 family interacting protein 1
Transcript:
RefSeq NM_030571.3 (NON-CURRENT)
Match location:
Position 1033 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATTTGTTCCTAGGTGTAAA
Hairpin Sequence:
5'-CCGG-CTATTTGTTCCTAGGTGTAAA-CTCGAG-TTTACACCTAGGAACAAATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATTTGTTCCTAGGTGTAAACTCGAGTTTACACCTAGGAACAAATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATTTGTTCCTAGGTGTAAACTCGAGTTTACACCTAGGAACAAATAG-3'

Other clones with same target sequence:

(none)