Construct: shRNA TRCN0000276210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018103.4-3396s21c1
DNA Barcode:
GCTTTAACTCCGTTCACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC8D (55144)
Gene Description:
leucine rich repeat containing 8 VRAC subunit D
Transcript:
RefSeq NM_018103.4 (NON-CURRENT)
Match location:
Position 3396 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAACTCCGTTCACTAAA
Hairpin Sequence:
5'-CCGG-GCTTTAACTCCGTTCACTAAA-CTCGAG-TTTAGTGAACGGAGTTAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAACTCCGTTCACTAAACTCGAGTTTAGTGAACGGAGTTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAACTCCGTTCACTAAACTCGAGTTTAGTGAACGGAGTTAAAGC-3'

Other clones with same target sequence:

(none)