Construct: shRNA TRCN0000276250

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003299.1-2538s21c1
DNA Barcode:
ATCCTGTGTGGAGAGGGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSP90B1 (7184)
Gene Description:
heat shock protein 90 beta family member 1
Transcript:
RefSeq NM_003299.1 (NON-CURRENT)
Match location:
Position 2538 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276250)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCTGTGTGGAGAGGGAATG
Hairpin Sequence:
5'-CCGG-ATCCTGTGTGGAGAGGGAATG-CTCGAG-CATTCCCTCTCCACACAGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCTGTGTGGAGAGGGAATGCTCGAGCATTCCCTCTCCACACAGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCTGTGTGGAGAGGGAATGCTCGAGCATTCCCTCTCCACACAGGAT-3'

Other clones with same target sequence:

(none)