Construct: shRNA TRCN0000276297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014655.2-636s21c1
DNA Barcode:
GCCAGAGTAACACAGTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A44 (9673)
Gene Description:
solute carrier family 25 member 44
Transcript:
RefSeq NM_014655.2 (NON-CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAGTAACACAGTCAAAT
Hairpin Sequence:
5'-CCGG-GCCAGAGTAACACAGTCAAAT-CTCGAG-ATTTGACTGTGTTACTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAGTAACACAGTCAAATCTCGAGATTTGACTGTGTTACTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAGTAACACAGTCAAATCTCGAGATTTGACTGTGTTACTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000127997