Construct: shRNA TRCN0000276330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016470.6-753s21c1
DNA Barcode:
TGTAAGAGATGGCATGATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSER1 (51526)
Gene Description:
oxidative stress responsive serine rich 1
Transcript:
RefSeq NM_016470.6 (NON-CURRENT)
Match location:
Position 753 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000276330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAAGAGATGGCATGATATG
Hairpin Sequence:
5'-CCGG-TGTAAGAGATGGCATGATATG-CTCGAG-CATATCATGCCATCTCTTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAAGAGATGGCATGATATGCTCGAGCATATCATGCCATCTCTTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAAGAGATGGCATGATATGCTCGAGCATATCATGCCATCTCTTACA-3'

Other clones with same target sequence:

(none)